Thursday, September 15, 2011

TECH 0708: Adsorption Column Chromatography Post-Lab

Post-Lab
2.  As hexane was added to the column, Acetylferrocene did progress down the column although very slowly.  It wasnt until TBME was added that acetylferrocene went down the column at a more rapid pace.
3.  Had hexane been the only eluent added to the column, the ferrocene would have come out of the column but since the acetylferrocene is more polar, it has a stronger attractive force to the alumna and wouldn't proceed down the column bed.
4.   If you had used TBME as the only eluent added, you wouldn't achieve seperation, both the ferrocene and acetylferrocene would proceed down the column bed together since ferrocene is more non-polar and holds less attraction to the absorbant,alumna. 

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