Results
I was assigned to do the Thin-Layer Chromatography on dichloromethane as a reagent for the first part of the lab. The middle set of dots on my TLC plate was due to error, while spotting the plate my capillary tube must have touched the TLC plate intentionally.
The distance between the origin and eluent front of dichloromethane was 6.0 cm.
From other team members TLC Plates: Hexane didn't move the eluent very far from the origin and didn't seperate so we decieded it is non-polar. Ethyl acetate and Methanol did move the eluent far up closer to the eluent front, thus showing that it is more polar, but also didn't seperate the compounds within the mixture. Toluene was the only other eluent that seperated the mixture of Biphenyl, Benzhydrol, and Benzophenone into it's individual compounds. So as a group we decided to use Toulene as being thMy assigned compound for this section of the lab was biphenyl. I spotted the stock solution on the left side of the TLC plate and biphenyl on the right side. After the plate had been developed, it appears that toluene takes much longer to evaporate than dicholoromethane. The distance between the origin and the topmost spot was 3.8 cm and the distance between the origin and the eluent front was 5.4 cm.
e best for seperating the mixtures later in this lab since my TLC plate had an error (the middle dots).
My unknown solution was #13 , and Toluene was used as the eluent. The left-most set of spots is the stock solution and the right-most set of spots is the unknown. Based on the results of the TLC plate, my unknown solution was comprised of Biphenyl and Benzhydrol. The distance between the origin and the eluent front is 5.8 cm, the distance between the origin and Biphenyl is 4.5 cm, and the distance between th origin and Benzhydrol is .5 cm.
No comments:
Post a Comment